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Compound 15 inhibits the <t>β‐catenin/TCF‐4</t> interaction. A coimmunoprecipitation assay was performed in HEK293T cells transiently transfected with FLAG‐tagged β‐catenin (β‐catenin FLAG ) and MYC‐tagged TCF‐4 (TCF‐4 MYC ). Cells were treated with 50 mM LiCl and 60 μM 15 for 24 h. β‐Catenin FLAG was immunoprecipitated from total protein extracts using anti‐FLAG‐conjugated agarose beads (left panel; IP: immunoprecipitation). Coimmunoprecipitated proteins were analyzed by Western blotting using an anti‐MYC‐tag antibody to detect TCF‐4 MYC (left panel; IB: immunoblot). Input shows the levels of TCF‐4 MYC and β‐catenin FLAG in 5% whole‐cell lysates before immunoprecipitation (right panel).
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Compound 15 inhibits the β‐catenin/TCF‐4 interaction. A coimmunoprecipitation assay was performed in HEK293T cells transiently transfected with FLAG‐tagged β‐catenin (β‐catenin FLAG ) and MYC‐tagged TCF‐4 (TCF‐4 MYC ). Cells were treated with 50 mM LiCl and 60 μM 15 for 24 h. β‐Catenin FLAG was immunoprecipitated from total protein extracts using anti‐FLAG‐conjugated agarose beads (left panel; IP: immunoprecipitation). Coimmunoprecipitated proteins were analyzed by Western blotting using an anti‐MYC‐tag antibody to detect TCF‐4 MYC (left panel; IB: immunoblot). Input shows the levels of TCF‐4 MYC and β‐catenin FLAG in 5% whole‐cell lysates before immunoprecipitation (right panel).

Journal: Chemmedchem

Article Title: 4‐(5‐Chloro‐3‐(3,4,5‐trimethoxybenzoyl)‐1 H ‐indol‐1‐yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β‐Catenin Signaling Pathway, and P‐Glycoprotein

doi: 10.1002/cmdc.202500996

Figure Lengend Snippet: Compound 15 inhibits the β‐catenin/TCF‐4 interaction. A coimmunoprecipitation assay was performed in HEK293T cells transiently transfected with FLAG‐tagged β‐catenin (β‐catenin FLAG ) and MYC‐tagged TCF‐4 (TCF‐4 MYC ). Cells were treated with 50 mM LiCl and 60 μM 15 for 24 h. β‐Catenin FLAG was immunoprecipitated from total protein extracts using anti‐FLAG‐conjugated agarose beads (left panel; IP: immunoprecipitation). Coimmunoprecipitated proteins were analyzed by Western blotting using an anti‐MYC‐tag antibody to detect TCF‐4 MYC (left panel; IB: immunoblot). Input shows the levels of TCF‐4 MYC and β‐catenin FLAG in 5% whole‐cell lysates before immunoprecipitation (right panel).

Article Snippet: HEK293T cells were transfected with pcDNA/Myc TCF‐4 (Addgene #16512) and human β‐catenin pcDNA3 (#16828 Addgene).

Techniques: Co-Immunoprecipitation Assay, Transfection, Immunoprecipitation, Western Blot